Parası kalmadığı için otobüse binemiyordur ailesi porno izle ona daha yeni para gönderdiği için tekrar porno istemeye utanınca mecburen otostop çekmek için youporn çantasını alarak yol kenarına gelir etekli porno liseli türk kız yol kenarında dururken yanına yaklaşan porno kibar bir gencin onu gideceği yere kadar bırakmak porno izle istemesine çok mutlu olur arabaya bindiklerinde gideceği yer ile porno arabayı kullanan adamın gittiği yer arasında çok mesafe sex izle farkı olduğunu anlayan türk kız bu yaptığı porno indir iyilik karşısında arabada ona memelerini açar porno sapıklaşan adam yol kenarındaki hotelde durarak porno izle üniversiteli otostop çeken türk kızına odada sakso çektirip sikerOptimized synthesis, characterization, antioxidative, antibacterial, anticancer cells proliferation and in vitro release kinetics study of chitosan and N, N, N-trimethyl chitosan nanoparticles loaded with Ocimum gratissimum essential oil| Abstract

7187379870

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Abstract

Optimized synthesis, characterization, antioxidative, antibacterial, anticancer cells proliferation and in vitro release kinetics study of chitosan and N, N, N-trimethyl chitosan nanoparticles loaded with Ocimum gratissimum essential oil

Author(s): Confidence Onyebuchi and Do??a Kavaz

The low solubility, loss of mucoadhesivity and poor absorption property of chitosan (CS) at physiological pH limits its applicability in biomedical and pharmaceutical field. N,N,NTrimethyl Chitosan (TMC) have shown enhanced penetration property, well-defined structure and improved solubility over wide pH range. Ocimum gratissimum Essential Oils (OGEOs) and methanolic extracts (OGEO-MeOH) have known bioactivity. Chemical qualitative analysis of the extracts by Gas Chromatography-Mass Spectrometry (GC-MS) showed newly found compounds not previously reported for OGEO such as eicosane, heneicosane, triphenylphosphine oxide, 1-acetyl2methyl-2-cycloppentene, (E)-9-octadecenoic acid, 2-carene, andgamma-terpinene. Different methods and optimized technique were adopted for the successful synthesis of OGEO-loaded Chitosan Nanoparticles (OGEO-CSNPs) and OGEO-loaded Trimethyl Chitosan Nanoparticle (OGEO-TMCNPs). With reference to zeta potential and polydispersity index of the nanoparticles. The synthesized nanoparticle was characterized with UV-Vis spectrophotometry, Fourier Transform Infrared Spectroscopy (FTIR) and scanning electron microscopy (SEM). In vitro-release kinetics of OGEO release revealed higher (P < 0.05) OGEO release efficiency from OGEO-TMCNPs over long period of time compared to the OGEO-CSNPs. The antioxidant activity assay showed that, OGEO-CSNPs and OGEO-TMCNPs never reached a steady state after 75 h. All samples exhibited antimicrobial properties at specific concentration. OGEO-TMCNPs exhibited antibacterial activity at lower concentration notably 40 mg mL-1 for E. coli, 20 mg mL-1 for B. cereus, 20 mg mL-1 for S. aureus and 80 mg mL-1 for S. typhimurium. In vitro cytotoxicity on MDA-MB-231 breast cancer cell lines revealed that OGEO-TMCNPs exhibited higher toxicity (P < 0.05). The physiochemical properties of OGEO-TMCNPs and OGEO-CSNPs have shown more promising application in pharmaceutical and food industries.


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