Parası kalmadığı için otobüse binemiyordur ailesi porno izle ona daha yeni para gönderdiği için tekrar porno istemeye utanınca mecburen otostop çekmek için youporn çantasını alarak yol kenarına gelir etekli porno liseli türk kız yol kenarında dururken yanına yaklaşan porno kibar bir gencin onu gideceği yere kadar bırakmak porno izle istemesine çok mutlu olur arabaya bindiklerinde gideceği yer ile porno arabayı kullanan adamın gittiği yer arasında çok mesafe sex izle farkı olduğunu anlayan türk kız bu yaptığı porno indir iyilik karşısında arabada ona memelerini açar porno sapıklaşan adam yol kenarındaki hotelde durarak porno izle üniversiteli otostop çeken türk kızına odada sakso çektirip sikerMaterials Technology 2019: Isoniazid-oleanolic acid co-crystal system: Synthesis, anti-TB and toxicological effect on the human embryonic kidney (HEK293) and human hepatocellular carcinoma (HepG2) cell lines- Victor O Fadipe, Federal Ministry of Science and Technology, Nigeria| Abstract

7187379870

All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Abstract

Materials Technology 2019: Isoniazid-oleanolic acid co-crystal system: Synthesis, anti-TB and toxicological effect on the human embryonic kidney (HEK293) and human hepatocellular carcinoma (HepG2) cell lines- Victor O Fadipe, Federal Ministry of Science and Technology, Nigeria

Author(s): Victor O Fadipe

 Tuberculosis (TB) is highly endemic disease worldwide. One of the commonly used first line drugs for anti-TB therapy (ATT) is isoniazid (INH). Isoniazid is known to be majorly metabolized and detoxified in liver by both phases I and phase II group of drug metabolizing enzymes. The drug and along with its metabolite are toxic and during its assimilation process cause injury to liver. The work presented here involves investigation of co-crystals involving isoniazid and oleanolic acid. A 1:1 co-crystal involving isoniazid, a foremost first-line drugs recommended by the World Health Organisation for the treatment of tuberculosis, which causes damage to the liver and Oleanolic acid, a hepatotoxicity naturally occurring compound, have been synthesized for the first time. Considering drug combination perspective, this is an interesting pharmaceutical co-crystal because of the known side effect of isoniazid therapy which might be improved upon by the presence of the oleanolic acid. The co-crystal compound was characterized using PXRD, TGA and SEM which were further evaluated for in vitro anti-TB and cytotoxicity index, using Human Embryonic Kidney (HEK 293) and Human Hepatocellular carcinoma (HepG2) cells. The PXRD of the synthesized co-crystal compound maintained crystalline nature like isoniazid for the three methods, TGA for all the three methods have cleavage values from 2200 C- 360O C, and the SEM images obtained from the three synthetic methods appears rod-like in nature. The co-crystal of OA with INH, increased the anti-TB MIC values for the three synthetic methods used as follows(a) Solvent evaporation(1.06μM) ;(b) Solvent drop (0.50μM) ;(c) Direct grinding (0.61 μM). The cytotoxicity tests of the co-crystal system on the two-human cell lines (HEK 293 and HepG2) were found to be IC50 ≥ 300ug/ml. The finding of the current work is that, co-crystal compound of OA with INH is probable and could be utilized to design better treatment for tuberculosis disease, which can further be extended to the treatment of HIV/TB


Share this       
Google Scholar citation report
Citations : 291

Materials Science: An Indian Journal received 291 citations as per Google Scholar report

Indexed In

  • CASS
  • Google Scholar
  • Open J Gate
  • China National Knowledge Infrastructure (CNKI)
  • Cosmos IF
  • Directory of Research Journal Indexing (DRJI)
  • Secret Search Engine Labs

Read More

Flyer