Current research on Jatropha Curcas L. is directed towards finding naturally- occurring antioxidants of plant origin that provided efficacy by additive or synergistic activities because antioxidants from plant origin are essential to prevent the progression of free radical mediated disorders. Antioxidant activity of Jatropha curcas L. bark extract (JCE) showed by using different in- vitro models. It includes Free radical scavenging activity of DPPH, Ferric reducing antioxidant power, Total flavonoid content and Total phenolic content. The plant contains much amount of Phenolic compounds and Flavonoids. Plant shows significant antioxidant activity.