The study investigated the anti-tumor activity of 50% aqueous ethanol extract of Acanthospermum hispidum DC against DaltonÂs ascites lymphoma in mice. The extracts were prepared by cold maceration with 50%aqueous ethanol and evaporated in vacuumto dry. (Yield: 50%aqueous ethanol -8.62%w/w). The extract were suspended in water with tween 20 and used for the study. The 50% aqueous ethanol extract (300 mg/kg p.o) was administered to tumor bearing mice (DAL) and examined for changes in dead cell count, histopathology of tumor cells, haematological parameters andmedian survival time (MST) and the results compared with that of tumor control or 5-FU. The findings reveal that 50% aqueous ethanol extracts possess anti-tumor activity. Thus it is suggested that Acanthospermum hispidum DC appears promising for the development of phyto-medicine for the treatment of cancer.