Simultaneous Estimation of Trifluoperazine Hydrochloride and Trihexyphenidyl Hydrochloride in Combined Tablet Dosage form by RP-HPLC Method

Author(s): Sunil L. Dabhade, A. Satishkumar Shetty, Manzoor Ahmed, B. Gopinath, B. K. Sridhar and Mithun L. Sureja

A new reverse phase high performance liquid chromatography (RP-HPLC) method has been developed for the simultaneous estimation of trifluoperazine hydrochloride and trihexyphenidyl hydrochloride in combined dosage form. A Inrtsil ods-3 C-18 column having dimensions of 250 × 4.6 mm and particle size of 5 µm, with mobile phase containing a mixture of acetonitrile : water : triethyelamine in the ratio of (68 : 31.8 : 0.2 v/v) was used. The pH of mobile phase was adjusted to 4.0 with orthophosphoric acid. The flow rate was 1 mL/min and the column effluents were monitored at 210 nm. The retention time for trifluoperazine hydrochloride and trihexyphenidyl hydrochloride was found to be 2.76 and 2.3 min, respectively. The proposed method was validated in terms of linearity, accuracy, precision, limit of detection, limit of quantitation and robustness. The method was found to be linear in the range of 10-150 µg/mL and 4-60 µg/mL for trifluoperazine hydrochloride and trihexyphenidyl hydrochloride, with regression coefficient r = 0.999, and r = 0.999), respectively.

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