Abstract

Simultaneous Estimation of Cilostazol and Aspirin in Synthetic Mixture using HPTLC Method

Author(s): Jayesh V. Patel, C. N. Patel, P. U. Patel, Pankaj H. Prajapati, I. S. Anand and M. B. Patel

A high performance thin layer chromatography (HPTLC) method was developed and validated for quantitative determination of cilostazol and aspirin in synthetic mixture. The analysis was performed using a mobile phase composed of methanol : ethyl acetate : toluene : solvent ether (1:2:2:0.5). The calibration graphs were linear correlation coefficient (r) > 0.995 in the studied concentration range of 100 to 800 ng/mL for cilostazole and 75-600 ng/mL for aspirin for HPTLC. Relative standard deviation of all the parameters for intraday and interday precision is less than 2% (0.75-1.25) and the accuracy was greater than 98%. So the proposed method is precise and accurate and can be applied directly and easily to the cilostazol and aspirin in synthetic mixture


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