Purification of Active Compounds from Kecapi Leaves that have Potential as Anticancer for In Vitro on Murine Cells Leukemia P-388

Author(s): Febria Elvy Susanti, Purwantiningsih Sugita and Laksmi Ambarsari

Kecapi is many contains secondary metabolites that can prevent a variety of diseases. The aims of this study were to obtain active compounds from the kecapi leaves through a purification, which includes vacuum liquid chromatography, gravity column chromatography, radial chromatography and preparative thin layer chromatography as well as the determination of the molecular structure and bioactivity as anticancer on murine cells leukemia P-388. Characterization of the isolated secondary metabolites is done by measuring the 1H-NMR and MS. The analysis showed that the isolated compounds suspected to contain (-)-Loliolide. Toxicity test using Brine Shrimp Lethality Test (BSLT) showed that E fraction was the most active fraction with IC50 of 2.03 x 10-17 μg mL-1. Anticancer test for in vitro on murine cells leukemia P-388 of methanol extract and E fraction result of vacuum liquid chromatography were performed the IC50 values decrease of 69.90 μg mL-1 to 13.86 μg mL-1 while bioactivity increased of 5 times.

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