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Estimation of Nevirapine in Tablets by HPLC

Author(s): K. Venugopal, G. Haritha, Y. Srinivasa Rao, K. E. V. Nagoji and J. V. L. N. Seshagiri Rao*

A reserved phase HPLC method is descnhed for the determination of ncvirapine in pharm aceutical dosage forms. Chromatography was carried out on an ODS column u sing a m ixture of accton i lrile and water (50:50 v/v) as the mobile phase at a fl ow rate of 1 .0 mUmin . N imesulide was used as an intern al standard and the detection was done at 230 nm using a UV detector. The retenti on time of the drug was 3.89 min . The method produced linear responses in the concentration range of 0.5 to 40 µg/mL of nevirapine. The method was found to be reprod ucible for analysis of the during in tabl ets.

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