Estimation of Mefenamic Acid in Pharmaceutical Dosage Forms by RP-HPLC

Author(s): R. Murali Krishna, M. Buela and A. Sahithi

A simple and precise RP-HPLC method was developed and validated for the determination of mefenamic acid in pharmaceutical dosage forms. Chromatography was carried out using an ODS packing L1, 250 x 4.6 mm, 5 µ,column at 27°C with a mobile phase acetonitrile : 0.05 M monobasic ammonium phosphate buffer : tetrahydrofuran (46 : 40 : 14) at a flow rate was 1.0 mL/min and UV detection wavelength was 254 nm. The retention time of the drug was 10.583 min. The proposed method was found to have linearity in the concentration range of 5-30 µg/mL with correlation coefficient of r2 = 0.99954. The developed method has been statistically validated and was found simple and accurate. The limit of detection and limit of quantification for mefenamic acid was found to be 0.12 and 0.36 µg/mL, respectively. The proposed method is accurate, precise, specific and rapid for estimation of mefenamic acid in tablet dosage form.

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