44 7460 854 031

All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Abstract

Development and Validation of RP- HPLC Method for the Determination of Acyclovir in Human Plasma

Author(s): Ch. Venkata Kumar, D. Anantahakumar and J. V. L. N. Seshagiri Rao

A rapid, selective and sensitive high performance liquid chromatographic method for the estimation of acyclovir in human plasma has been developed. Chromatography was carried out on a Hypersil BDS C18 column using a mixture of phosphate buffer (pH 2.5) and methanol in a ratio of 95 : 5 v/v as the mobile phase. The eluents were monitored at 254 nm. Lamivudine was used as an internal standard for this study. The retention times for acyclovir and lamivudine were found to be 5.5 and 8.0 min, respectively. The method was found to be linear in the concentration range of 0.201 to 13.545 AgmL-1. The method validated as per FDA guidelines and was found to be suitable for bioequivalence and pharmacokinetic studies.


Share this       
Google Scholar citation report
Citations : 9398

International Journal of Chemical Sciences received 9398 citations as per Google Scholar report

Indexed In

  • Google Scholar
  • Open J Gate
  • China National Knowledge Infrastructure (CNKI)
  • Cosmos IF
  • Geneva Foundation for Medical Education and Research
  • ICMJE

View More