Development and Validation of RP HPLC Method for the Estimation of Cefetamet in Bulk and Tablet Dosage Form

Author(s): N. Prudhvi, K. P. Channabasavaraj, Y. N. Manohara, A. L. Rao and D. B. Raju

A simple, rapid, sensitive and precise High Performance Liquid Chromatography (HPLC) method has been developed for the estimation of cefetamet in bulk and tablet dosage form. In this method, RP-C18 column (150 mm x 4.6 mm I.D., 5 7m particle size) with mobile phase consisting of acetonitrile, methanol and phosphate buffer (pH 3.60) in the ratio of 50 : 20 : 30 v/v/v in isocratic mode was used. The detection wavelength is 236 nm and the flow rate was 0.8 mL/min. In the range of 5-25 7g/mL, the linearity of cefetamet shows a correlation coefficient of 0.9995. The proposed method was validated by determining sensitivity, accuracy, precision and system suitability parameters. The method is simple, fast, accurate and precise and hence, it can be applied for routine analysis of cefetamet in bulk drug and its tablet dosage form.

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