Assessment of the protective role of hesperidin against genotoxic and biochemical effects of cytoxan in mice.

Author(s): A.Abd El Raouf , S.M.Girgis, H.S.Abdou, Eman M.Saleh

Hesperidin (HDN) is one of naturally occurring flavonoid widely found in citrus fruits. Flavonoidswere found to inhibitDNAand chromosomal damage induced by cyclophosphamide. Chemoprevention is one of the most promising and realistic approaches in the prevention of side effects produced by cytoxan treatment as an anticancer drug. Therefore, the present study was designed to evaluate the protective effect of HDN against genotoxic and biochemical effects of cytoxan (anti-tumor drug) in Swiss albino male mice. Mice were divided into 19 groups (5 animals each). The 1stgp served as control. The 2nd, 3 rd, 4th, gps had divided into 9 subgroups and received orally 50, 100 and 200mg/kg bw of cytoxan for one, two and three weeks, respectively. The 5th, 6th, 7th gps had divided into 9 subgroups and received 50, 100 and 200 mg/kg bw of cytoxan in combination with 50 mg ofHDNfor 1, 2 and 3weeks, respectively.At the end of the experimental periods, animals were subjected to genetic and biochemical analysis. The results revealed thatMNfrequency significantly increased in cytoxan treated mice. However, HDN treatment showed a significant decreament in MN frequency inmice bonemarrowcells compared to control.Aswell, a significant inhibition in DNA content using comet assay in cytoxan treated groups with 3 fold lower than control,whereas the treatmentwithHDN in improved that content. Also, there was a significant reduction in DNA, RNA and protein content in brain and kidney tissues and functions (creatinine and urea level elevation).However, administration of HDNin combinationwith cytoxan significantly improved all parameters studied in brain and kidney tissues.Asignificant decrease in the level of tissue antioxidants like superoxide dismutase (SOD), cholinesterase (CHE) and increase in malondialdhyde (MDA), cholesterol, glucose and triglycerollevelswas found in cytoxan treated groups. In conclusion, HDN supplementation significantly ameliorate these parameters due to antioxidant activity, thereby showing potent antigenotoxic and chemopreventive effects against toxicity induced by cytoxan in mice.

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