Nanotechnology (NT) is a field that is mushrooming, making an impact in all spheres of human life. Presently available literature revealed that the Titaniumdioxide (TiO2) synthesis usingmedicinal plants, microorganisms and algae and others as source has been unexplored and underexploited. NT is very important in developing sustainable technologies for the future, for humanity and the environment. The development of green processes for the synthesis of TiO2 NPs is evolving into an important branch of nanotechnology. Plantmediated synthesis of TiO2 nanoparticles is gaining more importance owing to its simplicity, rapid rate of synthesis of NP of attractive and diverse morphologies and elimination of elaborate maintenance of cell cultures and ecofriendliness. The nontoxic and biocompatible properties of Titania find its applications in biomedical sciences such as bone tissue engineering as well as in pharmaceutical industries. This review presents a summary of green synthesis and biocompatibility of and a concise account of the in vitro toxicity data on TiO2 nanoparticles. Presently, the researchers are looking into the development of cost-effective procedures for producing reproducible, stable and biocompatible metal NPs.