Activities of the distilledwater, ethanolic and chloroformextracts ofDaldina concentrica an ascomycetous fungus was investigated on Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa,Bacillus cereus and Staphylococcus aureus)using agarwell diffusionmethod. Ethanolic extract ofDaldina concentrica showed significantly antibacterial activity (P<0.05) against the test microorganisms except Bacilllus cereus. Staphylococcus aureus is the most sensitive organismto the extract of this fungus. Chloroformextract of Daldinia concentrica possessed higher anti -bacterial activity against the five tested microrganisms. The effect of fresh macro-fungus on test organisms was also studied. Fresh Daldina concentrica was more active against Proteus mirabilis in comparison witn other pathonenic microogansms.