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Immunhistochemistry

  Immunohistochemistry (IHC) is that the commonest application of immunostaining. It involves the method of selectively identifying antigens (proteins) in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues. Immunohistochemistry (IHC) is a crucial application of monoclonal also as polyclonal antibodies to work out the tissue distribution of an antigen of interest in health and disease. IHC is widely used for diagnosis of cancers; specific tumour antigens are expressed de novo or up-regulated in certain cancers. Immunohistochemistry (IHC) may be a powerful microscopy-based technique for visualizing cellular components, as an example proteins or other macromolecules in tissue samples. The strength of IHC is that the intuitive visual output that reveals the existence and localization of the target-protein within the context of various cell types, biological states, and/or subcellular localization within complex tissues. The IHC technique was invented during the 1940s (Coons, Creech, & Jones, 1941) and is routinely used as an important tool in health care and pathology for e.g. diagnostic purposes or to stratify patients for optimized treatment regimes. IHC is also widely used in research where molecules of interest are analysed to study their roles in both healthy and diseased cells and tissues on the molecular, cellular or tissue level. 

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