Parası kalmadığı için otobüse binemiyordur ailesi porno izle ona daha yeni para gönderdiği için tekrar porno istemeye utanınca mecburen otostop çekmek için youporn çantasını alarak yol kenarına gelir etekli porno liseli türk kız yol kenarında dururken yanına yaklaşan porno kibar bir gencin onu gideceği yere kadar bırakmak porno izle istemesine çok mutlu olur arabaya bindiklerinde gideceği yer ile porno arabayı kullanan adamın gittiği yer arasında çok mesafe sex izle farkı olduğunu anlayan türk kız bu yaptığı porno indir iyilik karşısında arabada ona memelerini açar porno sapıklaşan adam yol kenarındaki hotelde durarak porno izle üniversiteli otostop çeken türk kızına odada sakso çektirip sikerSuperoxide Dismutase Based Biosensor for the Electrochemical Determination of Epinephrine| Abstract

Abstract

Superoxide Dismutase Based Biosensor for the Electrochemical Determination of Epinephrine

Author(s): Miriam Barquero-Quirós*, Hugo Cunha-Silva and María Julia Arcos-Martínez

New amperometric biosensor for epinephrine was developed, using a screen printed carbon electrode with tetrathiafulvalene (5% v/w) incorporated into carbon ink (SPCTTFE). Electrodic surface was modified with nanoparticles of Au, Pt, Pd and Rh deposited on SPCTTFE by cyclic voltammetry when using Pd nanoparticles, slope of EPI calibration curves by was higher than the performed with the others NPs. The electrodeposited nanoparticles were evaluated with atomic force microscopy and electrochemical impedance spectroscopy was used to characterize electrode process. The developed superoxide dismutase-based biosensor was characterized by: limit of detection of 5.3 × 10-6 M (n=4), limit of quantification of 17.5 × 10-6 M (n=4), reproducibility with RSD of 2.8% (n=5), repeatability with RSD of 0.97% (n=3), accuracy was 102.8% with RSD of 4.3% (n=5). Linearity was obtained from 17.0 × 10-5 M to 8.59 × 10-4 M. Interference study performed adding ascorbic acid and uric acid exhibits that the peak potential of both species are higher than the chosen for EPI analysis. Therefore developed biosensor and described in this paper, has been successfully applied to the determination of I in human gamma globulin and pharmaceutical samples. Developed biosensor offers easy assembly, excellent linearity and good performance parameters.


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Citations : 748

Acta Chimica and Pharmaceutica Indica received 748 citations as per Google Scholar report

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