Spectrophotometric Quantification of Balofloxacin using Multivariate Technique in Pharmaceutical Formulation

Author(s): Krishna Kishore Ch, J. Kavitha, L. Bona Venture Michael and K. S. Lakshmi

The study was focused to develop and validate a simple, sensitive and accurate UV spectrophotometric method with multivariate calibration for the quantification of balofloxacin in pharmaceutical dosage form. This technique was based on the use of the linear regression equations by using relationship between concentration and absorbance at five different wavelength sets. The developed method was validated for accuracy, precision and sensitivity according to ICH guidelines. The plot was found to be linear in the concentration range of 10-50 μg/mL with significantly high value of correlation coefficient. The linear regression analysis data for the calibration plots showed good linear relationship with R2 = 0.9999. The regression equation was found to be Y = 0.0114 X + 0.0885. The LOD and LOQ were found to be 1.38 and 4.11 μg/mL, respectively and the method was found to be sensitive. This statistical approach gives optimum results for eliminating fluctuations coming from instrumental or experimental conditions and it also has considerable sensitivity, rapid, accuracy and low cost for the quantitative analysis, quality control and routine analysis of balofloxacin in pharmaceutical formulation.

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