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Simultaneous Spectrophotometric Estimation of Nebivolol Hydrochloride and Hydrochlorothiazide in Combined Tablet Dosage form by Area Under Curve Method

Author(s): Jignesh S. Shah, Satishkumar A. Shetty, Manzoor Ahmed, B. K. Sridhar and Dharmesh J. Jani

The objective of the current study was to develop a simple, accurate, precise and rapid UV spectrophotometric method with subsequent validation using ICH suggested approach for the determination of antihypertensive pharmaceutical dosage form containing binary mixture of nebivolol hydrochloride (NEB) and hydrochlorothiazide (HCZ) using methanol as the solvent. The proposed area under curve method involves the measurement of area at selcted analytical wavelength ranges and performing the analysis using “Cramer’s Rule” and “Matrix Method”. Two analytical wavelength ranges selected were 295-285 nm and 312-322 nm for the estimation of NEB and HCZ. The linearity of the proposed method was investigated in the range of 5-70 Hg/mL (r = 0.9998) for NEB and 5-70 Hg/mL (r = 0.9998) for HCZ, respectively. The percentage mean recovery was found to be 98.90% for NEB and 99.26% for HCZ. Also the method was statistically validated for its linearity, accuracy and precision. Both inter-day and intra-day variation was found to be showing less % RSD value indicating high grade of precision of the method.

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