Simultaneous Spectrophotometric Estimation of Atorvastatin Calcium and Amlodipine Besylate in Combined Tablet Dosage form by Area Under Curve Method

Author(s): Dharmesh J. Jani, Manzoor Ahmed, Satishkumar A. Shetty, B. K. Sridhar and Jignesh S. Shah

The objective of the current study was to develop a simple, accurate, precise and rapid UV spectrophotometric method with subsequent validation using ICH suggested approach for the determination of atorvastatin calcium (ATR) and amlodipine besylate (AML) using methanol as the solvent. The proposed area under curve method involves the measurement of area at selected analytical wavelength ranges and performing the analysis using “Cramer’s Rule” and “Matrix Method”. Two analytical wavelength ranges selected were 256-238.5 nm and 368-352 nm for the estimation of ATR and AML. The linearity of the proposed method was investigated in the range of 5-50 μg/mL (r = 0.9998) for ATR and 5-50 μg/mL (r = 0.9997) for AML, resptectively. The percentage mean recovery was found to be 99.83% for ATR and 99.60% for AML. Also the method was statistically validated for its linearity, accuracy and precision. Both inter-day and intra-day variation was found to be showing less % RSD value indicating high grade of precision of the method.

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