Simultaneous Spectrophotometric Estimation of Amoxicillin and Carbocisteine by Second Order Derivative Spectroscopy Method in Combined Dosage FormAuthor(s): Rajan V. Rele
The objective of the study was to develop a simple, accurate, precise and rapid UV spectrophotometric, second order derivative method fo r validation of amoxicillin and carbocisteine. The validation was carried out by using ICH guidelines for the determination of amoxicillin and carbocisteine by using 0.1 N hydrochloric acid as the solvent in combined dosage form. The proposed second order derivative method involves the measurement of absorb ance of one drug at zero crossing point of other; hence, wavelengths 241.8 nm and 208 nm were selected for the estimation of amoxicillin and carbocisteine, respectively. The linear ity of the proposed method was found in the concentration range of 20 to 100 μg/mL (r 2 = 0.9998) for amoxicillin and 10 to 100 μg/mL (r 2 = 0.9988) for carbocisteine, respectively. The percentage mean recovery was found to be 100.129% for amoxicillin and 100.163% for carbocisteine, respectively. The method was also sta tistically validated for its linearity, accuracy and precision. Both intra- and inter-day variations showed less percentage (%) RSD values indicating high grade of precision of this method.