Simultaneous Estimation of Ofloxacin and Tinadazole by Reverse Phase High Performance Liquid Chromatography

Author(s): S. Appala Raju, P. N. Sanjay Pai, Khaja Pasha and S. Humaira

An HPLC method for simultaneous estimation of ofloxacin and tinidazole was developed using SS wakosil II. C18, 250 x 4.6 mm, 5 μm column. With mobile phase composition of acetonitrile and phosphate buffer 3 : 1 (pH 5), flow rate of 1.0 mL/min and UV detection at 295 nm linearity was observed over concentration range of 10-50 μg/ mL for ofloxacin and 10-80 μg/ mL for tinidazole. The accuracy of the proposed method was determined by recovery studies and found to be 95-105% for ofloxacin and 101-103% for tinidazole, the proposed method was validated and results conformed with the ICH parameters.

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