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Simultaneous Determination and Method Validation of Ceftriaxone Sodium and Sulbactam Sodium by Reverse Phase Ion - Pair HPLC

Author(s): Rajesh Sharma, Nita Yadav, Ganesh P. Mishra and Subhash C. Chaturvedi

A simple, precise, accurate, rapid and reproducible reverse phase ion-pair high performance liquid chromatographic procedure was developed for simultaneous determination of ceftriaxone sodium and sulbactam sodium in sterile powder for injection. The mobile phase used was a combination of acetonitrile: 0.03M phosphate buffer (55:45,v/v) and the pH was adjusted to 5.0 by addition of triethylamine, 3.2 g/L tetraheptyl ammonium bromide was added as ion-pair reagent. The detection of the combined dosage form was carried out at 230 nm and a flow rate was set to 1 mL/min. Linearity was obtained in the concentration range of 50.0 to 400.0 µg/mL of ceftriaxone sodium and 25.0 to 200.0 µg/mL of sulbactam sodium with co-relation coefficient of 0.9999 and 0.9998, respectively. The results of the analysis were validated statistically and recovery studies confirmed the accuracy of the proposed method.

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