All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Abstract

Simultaneous Determination and Method Validation of Ceftriaxone Sodium and Sulbactam Sodium by Reverse Phase Ion - Pair HPLC

Author(s): Rajesh Sharma, Nita Yadav, Ganesh P. Mishra and Subhash C. Chaturvedi

A simple, precise, accurate, rapid and reproducible reverse phase ion-pair high performance liquid chromatographic procedure was developed for simultaneous determination of ceftriaxone sodium and sulbactam sodium in sterile powder for injection. The mobile phase used was a combination of acetonitrile: 0.03M phosphate buffer (55:45,v/v) and the pH was adjusted to 5.0 by addition of triethylamine, 3.2 g/L tetraheptyl ammonium bromide was added as ion-pair reagent. The detection of the combined dosage form was carried out at 230 nm and a flow rate was set to 1 mL/min. Linearity was obtained in the concentration range of 50.0 to 400.0 µg/mL of ceftriaxone sodium and 25.0 to 200.0 µg/mL of sulbactam sodium with co-relation coefficient of 0.9999 and 0.9998, respectively. The results of the analysis were validated statistically and recovery studies confirmed the accuracy of the proposed method.


Share this       
Google Scholar citation report
Citations : 9398

International Journal of Chemical Sciences received 9398 citations as per Google Scholar report

Indexed In

  • Google Scholar
  • Open J Gate
  • China National Knowledge Infrastructure (CNKI)
  • Cosmos IF
  • Geneva Foundation for Medical Education and Research
  • ICMJE

View More