RP-HPLC Method for Estimation of Paracetamol from Pharmaceutical Formulation Febrinil

Author(s): P. R. Solanki, S. Prachi and S. D. Boob

A simple, rapid and sensitize high performance liquid chromatography method for determination of paracetamol in a commercial dosage from Febrinil has been developed. The SHIMADZU LC 20 AT dual pump chromatograph system used a reverse phase C-18, column phenomenex C 18 5 μ, 250 mm x 4.6 mm, utilizing a mobile phase of acetonitrile / water (60 : 40 v/v/) at a flow rate of 1 mL/ min with UV detection at 210 nm. The retention time for paracetamol was found to be 2.690 min. and recovery from the formulation was 90%. The developed method was validated in terms of accuracy, precision, linearity, limit of detections, limit of quantification. This method can be used for estimation of paracetamol in pharmaceutical formulation.

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