Qualitative Estimation of Nevirapine in Pharmaceutical Dosage Forms by RP-HPLC

Author(s): Y. Padmanabha Reddy, P. Sreekant and H. G. Raghavendra

A simple, precise, rapid and reproducible method has been developed and validated for estimation of nevirapine in pharmaceutical dosage forms by RP-HPLC. The separation was achieved using SUPELCOSIL LC-ABZ end capped (5 μm) 150 x 4.6 mm column in isocratic mode with mobile phase a mmonium phosphate buffer : acetonitrile(4 : l) were flushed at a flow rate of l.0 mL/min. Detection was carried out at 220 nm.The retention time for nevirapine was 7.347 min. The percentage recovery was found to be 99.0-101.0%w/w.

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