Abstract

Optimization of Glucose Syrup Production Process using Sabrang Potato Starch (Coleus tuberosus Benth) by Hydrolysis Enzimatic

Author(s): Yunianta, Widya Dwi Rukmi P and Suburi Rahman

The enzymatic hydrolysis of starch is one of many options in the food and beverage industry in particular in the manufacture of glucose syrup. In this study the effect of time and the use of saccharification enzyme glucoamylase concentrations studied and optimized to produce glucose syrup from sabrang potato starch (Coleus tuberosus Benth). There are two variables used in this study with three levels in each variables. The first variable is saccharification time which are 12, 24 and 36 hours. The second variable is glucoamylase concentrations which are 3.00, 4.50 and 6.00mg / ml. The results in the hydrolysis of starch potato sabrang enzymatically analyzed using Design Expert software version 7. Statistical significance of the model is validated by the F-test for analysis of variance (p ï‚£ 0.05). The results showed that the optimum conditions for saccharification time of 27 hours 49 minutes, and glucoamylase concentration of 4.96 mg / ml. The maximum production at optimum conditions produced glucose levels by 22.69%, with the value of dextrose equivalent as much as 89.11% and produced 75.63%of glucose.