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Method Development and Validation of RP-HPLC Method for Famciclovir

Author(s): Swapna Velivela, Konde Abbulu, Vinyas Mayasa, Nikunja Pati, Gopi Yadav and V. R. M. Gupta

A high-performance liquid chromatographic method for the determination of famciclovir, a highly active antiviral agent, was developed in the present work. The various parameters, such as linearity, precision, accuracy, specificity, robustness, limit of detection and limit of quantitation were studied according to International Conference on Harmonization guidelines. HPLC was carried out by using the reversed-phase technique on an RP-C18 column with a mobile phase composed of methanol and water (75 : 25 v/v), adjusted to pH 3.05 with orthophosphoric acid. The mobile phase was pumped at a flow rate of 1 mL/min and detection was made at 221 nm with UV dual absorbance detector. The proposed methods are highly sensitive, precise and accurate and therefore can be used for its intended purpose.

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