in vitro Antioxidant and Free Radical Scavenging Activity of Eugenia Bracteata RoxbAuthor(s): E. Hemalatha, T. Satyanarayana and G. Alekhyanjali
The role of free radicals has been implicated in the causation of several diseases such as liver cirrhosis, atherosclerosis, cancer, aging, arthritis, diabetes, etc. Eugenia bracteata Roxb also known as Jaman has been useful in the treatment of diabetes. There is no detailed study on free radical scavenging activity on Eugenia bracteata. Hence, a detailed study was carried out on the methanolic extract of Eugenia bracteata for scavenging activity of superoxide radical, hydroxyl radical, DPPH radical, hydrogen peroxide, nitric oxide, and hypochlorous acid scavenging activities and lipid peroxidation inhibition. In the present study Eugenia bracteata showed free radical scavenging activity on wide range of free radicals. The results are statistically comparable with standard reference ascorbic acid. The mean IC50 values for scavenging activity by methanolic extract of Eugenia bracteata were found to be 198.406 μg (superoxide), 538.336 μg (hydroxyl), 1145.076 μg (lipid peroxidation), 22.3725 μg (DPPH), 123.276 μg (nitric oxide), 11.7 μg (hypochlorous acid), and 88.148 μg (hydrogen peroxide). Eugenia bracteata showed potent scavenging activity on superoxide; equal effect on hydrogen peroxide and hypochlorous acid; moderate effect on DPPH; low activity on hydroxyl radical; inhibition of lipid peroxidation and nitric oxide compared to standard ascorbic acid.