Abstract

Evaluation of the antioxidant capacity of gallic acid encapsulated in liposomes

Author(s): T.A.Munoz Quinones, J.A.Gallegos Infante , N.E.Rocha Guzman, R.F.Gonzalez-Laredo,L.Medina Torres, H.F.Casanova Yepez

The protection and improvement of bioavailability of phenolic compounds is a problem that can be solved by various methods including the use of liposomes. The aim of this study was to determine the effect of gallic acid concentration on the entrapment efficiency by liposomes and to determine their antioxidant capacity. Liposomes were produced at three different concentrations of gallic acid (10, 100 1000g/mL), gallic acid was determined using the Folin- Ciocalteumethod (Previous, sampleswere separated chromatographically), the antioxidant capacity was evaluated by DPPH (1,1- diphenyl-2-picrylhydrazyl) method and the determination of the empirical constant of breakdown chain speed and efficiency of entrapment of gallic acid. The results obtained indicate that the highest content of gallic acid was found in liposomes with 100g/mL, also the increased efficiency of entrapment was associated with this concentration (98%). The ability of free radical trapping showed no difference between the liposomeswith 100 and 1000g/mL. The higher speed of chain breakage was found in the liposomes at 1000g/mL. Results indicate that it is possible to obtain liposomes with gallic acid at very high efficiency.