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Estimation of Palonosetron Hydrochloride (A 5-HT3 Antagonist) in Pharmaceutical Dosage Form by U.V. Spectrophotometric Method

Author(s): Della Grace Thomas Parambi, SR. Molly Mathew and V. Ganesan

A simple, rapid, economical, accurate and precise method has been developed for estimation of palonosetron hydrochloride from tablet dosage form. The absorption maxima in ethanol solvent was found to be at 265 nm and Beer’s law was obeyed in a concentration range of 7.5-25 μg/mL and coefficient of correlation for palonosetron was found to be 0.9997. The precisional accuracy of the developed method was confirmed by repeatability and recovery studies are validated statistically. The percentage recovery was found to be 99.3% for palonosetron. Standard sensitivity of the above method was 0.0075 μg. This method showed good repeatability and recovery with relative standard deviation less than 2. So, this developed method can be used for the routine analysis of palonosetron from formulations

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