Escherichia coli Co-Expressing Lipoxygenase: Highly Efficient Oxidation of Plant Oils to C18 Trihydroxy Fatty Acids

Author(s): Eric J. White

A C18 Tri Hydroxyl Adipose Acids (THFAs) industrial facility oxy lipids are utilized as antifungal specialists and immunization adjuvants. The compound conflation of THFAs has a few disservices including low yields and ecological contamination. Microorganisms and shops additionally produce THFAs in vivo; still the efficiency is low. Then, we revealed a recombinant Escherichia calico-communicating bacterial Linoleic Acid (LA) 13-lipoxygenase with high Hydroxyl Adipose Acids effort and epoxide hydrolase that changed over 200 mm polyunsaturated fat acids including LA α-linoleic corrosive, and γ-linoleic corrosive (GLA) into THFAs through library glue hydroxyl fat acids with high transformation yields (>60) in a measuring utensils. Among the items GLA-concluded library glue 11Rhydroxyoctadecadienoic corrosive and trihydroxyoctadecadienoic corrosive were new composites. For the successful biotransformation of safflower material into THFA the LA content in the safflower material hydrolyzed was expanded by the expansion of an adsorbent tar with lipase to safflower material.

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