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DNA Barcoding and Sequence Annotation Study of Diplokhema butyracea Spp. (Chiuri) from Nepal

Author(s): Deepak Sharma*, Tirtha Maiya Shrestha and Janardan Lamichhane

Diplokhema butyracea spp. (Chiuri), also known as the butter tree, is a Himalayan medicinal plant of Nepal with high economic value. The use of molecular genetic method of DNA barcoding for the authentication and studies of these plants in specific sites can have a positive impact on the use of raw materials from this plant in herbal medicinal products. The Internal Transcribed Spacer (ITS) gene locus been shown to be the most effective source of DNA barcodes for this plant. Using Basic Local Alignment Search Tool (BLAST) searching of GenBank and Neighbor Joining (NJ) analysis shows very close sequence similarities (99.4%) with the other species of Diplokhema (National Center for Biotechnology Information (NCBI) accession number MT497981) from other countries. As this medicinal plant has been used as an important herbal drug ingredient, the use of this marker gene (ITS) can allow researchers to identify material from even highly degraded DNA samples or in the adulterants from herbal products. Accurate identification of this material is especially important in the case of herbal preparations and for biologically active additives including studies of heavy metal or minerals in the future.

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