Parası kalmadığı için otobüse binemiyordur ailesi porno izle ona daha yeni para gönderdiği için tekrar porno istemeye utanınca mecburen otostop çekmek için youporn çantasını alarak yol kenarına gelir etekli porno liseli türk kız yol kenarında dururken yanına yaklaşan porno kibar bir gencin onu gideceği yere kadar bırakmak porno izle istemesine çok mutlu olur arabaya bindiklerinde gideceği yer ile porno arabayı kullanan adamın gittiği yer arasında çok mesafe sex izle farkı olduğunu anlayan türk kız bu yaptığı porno indir iyilik karşısında arabada ona memelerini açar porno sapıklaşan adam yol kenarındaki hotelde durarak porno izle üniversiteli otostop çeken türk kızına odada sakso çektirip sikerDevelopment of Novel 1,3,5-Triazene-cored maltoside amphiphiles for membrane protein extraction and stabilization| Abstract

Abstract

Development of Novel 1,3,5-Triazene-cored maltoside amphiphiles for membrane protein extraction and stabilization

Author(s): Lubna Ghani

 Despite the great importance of membrane proteins, structural studies of these proteins present a major challenge. A significant issue associated with these proteins is to isolate them in a stable and functional state from natural membranes of lipid bilayers. Detergents are usually used to extract these proteins from the lipid bilayers and maintain them in a soluble and stable state in aqueous medium. However, due to limitations of conventional detergents it is essential to develop novel amphiphiles with enhanced membrane protein stability for advancing membrane protein research. Here we designed and synthesized 1,3,5-triazene-cored dimaltoside amphiphiles derived from cyanuric chloride. By introducing variations in the alkyl chain linkage (ether/thioether) and an amine-functionalized diol linker (serinol/diethanolamine), we prepared two sets of novel detergents. When tested with a few model membrane proteins, these agents showed remarkable efficacy in stabilizing the membrane proteins. Detergent efficacy for protein stabilization substantially varied depending on detergent structural variation, allowing us to discuss detergent structure-property-efficacy relationship. The triazene-based detergents introduced here hold significant potential in membrane protein study because of their structural diversity and universal stabilization efficacy toward multiple membrane proteins. 

 


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