Abstract

Development and Validation of RP-HPLC Method for Simultaneous Determination of Cefotaxime Sodium and Sulbactum Sodium in Bulk and Injection

Author(s): Rohan S. Badgujar, Saurabh Kumar Banerjee and G. Venkateshwarlu

A reverse phase high performance liquid chromatographic method was developed for simultaneous determination of cefotaxime sodium and sulbactum sodium in bulk and injection. The separation was made by a Phenomenex Gemini C18 column (250 cm × 4.6 mm, 5 μm) using buffer (40% tetrabutyl ammonium hydrochloride) : acetonitrile : methanol (8 : 1.75 : 0.25, v/v/v) as mobile phase. The validation of the method was performed, and specificity, reproducibility, precision and accuracy were confirmed. The limit of detection was approximately 0.57 μg/mL for cefotaxime sodium and 0.49 μg/mL for sulbactum sodium. Due to simplicity and accuracy, the method is particularly suitable for routine pharmaceutical quality control.


Share this       
izmir escort izmir escort bursa escort antalya escort izmir escort porno porno izle türk porno eskişehir escort bartın escort burdur escort izmir escort bursa escort porno indir izle escort izmir