Determination of Olmesartan and Hydrochlorthiazide in Pharmaceutical Formulations by RP-HPLC

Author(s): V. Amudhavalli, K. S. Lakshmi and M. Karthick

A simple and sensitive reverse phase high perf ormance liquid chromatographic method for the determination of Olmesartan (OLM) and Hydrochlorthiazide (HCZ) was developed on a Shimadzu Class vp series HPLC system with a phenomenex C 18 column (150 x 4.6 mm, 5 μ) using a mobile phase mixture containing acetonitrile and ammonium acet ate buffer (pH-3.5) in the ratio of 55 : 45. The flow rate was 1.0 ml/min and effluents were monitored at 252 nm and eluted at 2.14 min (OLM) and 4.62 min (HCZ). Calibration curve was plotted with a range from 2.0-20 μg/mL for OLM and 1.0 -10 μg/mL for HCZ. The assay was validated for the parameters like accuracy, precision, robustness and system suitability parameters. The results were found to be satisfactory and the method can be adapted for the routine quality control of the drugs in formulations.

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