Parası kalmadığı için otobüse binemiyordur ailesi porno izle ona daha yeni para gönderdiği için tekrar porno istemeye utanınca mecburen otostop çekmek için youporn çantasını alarak yol kenarına gelir etekli porno liseli türk kız yol kenarında dururken yanına yaklaşan porno kibar bir gencin onu gideceği yere kadar bırakmak porno izle istemesine çok mutlu olur arabaya bindiklerinde gideceği yer ile porno arabayı kullanan adamın gittiği yer arasında çok mesafe sex izle farkı olduğunu anlayan türk kız bu yaptığı porno indir iyilik karşısında arabada ona memelerini açar porno sapıklaşan adam yol kenarındaki hotelde durarak porno izle üniversiteli otostop çeken türk kızına odada sakso çektirip sikerChemical crosstalk between plants and microorganisms: Camptothecin and maytansine as an example| Abstract

Abstract

Chemical crosstalk between plants and microorganisms: Camptothecin and maytansine as an example

Author(s): Michael Spiteller

Backgroud: Studies on microbe-host interactions in plant aimed at understanding the role of these associations and their utility in pharmaceutical and agricultural sectors are gaining impetus. Several recent studies have lent evidence to the fact that certain so-called “plant metabolites” are actually biosynthesized by associated endophytic microorganisms.

Aims: We wanted to elucidate the biosynthesis of the important anticancer drug maytansine in Celastraceae plants in order to elucidate its actual producer(s), which has been an open question since its discovery in the 1970s.

Results: We showed that maytansine is actually a biosynthetic product of root-associated endophytic bacterial community in Putterlickia verrucosa and Putterlickia retrospinosa plants. This interesting outcome provided the scientific basis to investigate the actual producer(s) responsible for maytansine biosynthesis in Maytenus plants. Endophytic communities harboring different tissues of Maytenus serrata originating from Cameroon were investigated using a combination of bioanalytical tools such as HPLC-HRMSn and MALDI-MSI, and targeted genome mining techniques to elucidate the source and sites of maytansine biosynthesis. We proved that the biosynthesis of maytansine in M. serrata is shared between the endophytic bacterial community colonizing the stem and the host plant containing non-culturable cryptic endophytes.

Conclusion: Our work demonstrates that maytansine is biosynthesized in M. serrata only when the host plant joins forces with its selected and very eco-specific endophytic bacterial community.


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