A simple, specific, accurate, precise and sensitive Reverse phase high performance liquid chromatographic method has been developed for the quantitation of Eprosartan mesylate in both pure and pharmaceutical dosage forms. An Phenomenex Luna 5 μ C-18(2) 100A column having 250 x 4.6 mm internal diameter in isocratic mode with mobile phase containing Acetonitrile : 1% Diethyl amine : 1% Glacial acetic Acid (13 : 3 : 4 v/v/v). The flow rate was 0.6 mL/min and the effluents were monitored at 242 nm. The retention time was 4.757 min. The linearity was in the range of 5-20 μg/mL. This method was validated for linearity, precision, limit of detection, limit of quantitation and accuracy. Statistical analysis proves that the method is reproducible and selective for the estimation of the said drug.