Use of Spectrophotometric Method and Flow Injection Techniques for Determining Copper (II) by 2-(4-Bromo 2,6-Dimethyl Phenyl) Azo 4,5-Diphenyl-1-Imidazol)

Author(s): Kheedija Jabbar Ali, Abid Allah Mohammed Ali and Haider Norri Raheem

This research includes use of an organic reagent 2-(4-bromo 2,6-dimethyl phenyl) azo 4,5-diphenyl-1-imidazol) for determination of Cu(II) ion by spectrophotometric method. After determining the optimum conditions of the complex reaction such as pH value, reagent concentration and the results were 8.0 and 10.0 × 10-4 M, respectively and the temperature and equilibrium time of complex determination too. The stoichiometry of complex was determined by methods (mole ratio and Job method) and it was (2:1) (reagent : ion). Then calibration graph was prepared. The linearity (7 × 10-5 – 10-6 M) sensitivity 5261.2 and detection limit of Cu(II) complex was found by depend on the calibration graph. The effect of foreign ions was studied and suitable the masking agents were found for each. The new reagent was used for determination Cu(II) ion in new design of flow injection system (FIA). Various parameters, physical and chemical, affecting the determination have been investigated such as flow rate, reaction coil, volume reagent, volume sample pH and reagent concentration. The results were 14 mL/min, 15 cm, 0.253 mL, 0.253 mL, 8, 5 × 10-5 M, respectively then preparation the calibration graph in FIA system and the average of dispersion coefficient determined reliability were studied. The results were compared between two methods (spectrophotometric method and FIA method

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