In this study, a biological evaluation of the antimicrobial activity of copolymer of styrene and acetyl thiophene containing uranyl nitrat e initiated by BPO was carried out using Escherichia coli (Gram negative) and Staphylococcus aureus (Gram positive) as model organisms. BPO is free radical initiator so copolymer was prepared by free radical polymerisation. The physical properties such as softening range, solubility, permeability and adsorption of the synthesized polymer films were checked in laboratory. Preparation of polymer films were confermed by IR and NMR spectroscopy. The antibacterial activity of copolymer films against bacteria were investigated by calculation of minimum inhibitory concentrations. These investigations reveal that the highest conc entration of acetyl thioph ene and metal show the minimum growth of S. aureus and E. coli .