A simple, sensitive and resproducible spectrophotometric method for the analysis of glimepiride in pure form and in pharmaceutical formaulations has been developed. glimepiride is a synthetic oral hypoglycemic agent, which belongs to the class of sulfonylureas used in treatment of type- 2 diabetes mellitus. Glimepiride exhibited maximum absorbance at 226.0 nm with an apparent molar absorptivity of 2.94 x 106. Beer’s law was obeyed in the concentration range of 2-32 μg/mL. Results of the analysis were validated statistically and by recovery studies. This method is successfully employed for the determination of glimepiride in various pharmaceutical preparations.