A simple, accurate and precise reverse phase high performance liquid chromatographic method has been developed and validated for the estimation of satranidazole and ciprofloxacin simultaneous determination in combined dosage forms. A Lichrospher 100 RP-180, C18 column was used as stationary phase and mobile phase contain water : Acetonitrile : triethylamine (75 : 25 : 0.1, v/v/v) and final pH adjusted to 3.25 ± 0.02 with 10 % v/v o-phosphoric acid. Measurements were made at the effluent flow rate of 1.0 mL/min with injection volume 20 :l and ultraviolet (UV) detection at 320 nm, as both components shows reasonable good response at this wavelength. The retention times of satranidazole and ciprofloxacin were 5.38 min and 3.31 min, respectively. The method was validated in terms of linearity, accuracy, precision, robustness and specificity. Linearity of satranidazole and ciprofloxacin was in the range of 1-70 :g/mL for both, respectively. Average percentage recoveries obtained for satranidazole and ciprofloxacin were 100.14 % and 101.03 %, respectively. The limit of detection and limit of quantification were found to be 0.3 and 1.0 μg/mL for satranidazole, respectively and for ciprofloxacin were 0.6 and 1.0 μg/mL, respectively. The method is useful in the quality control of bulk manufacturing and pharmaceutical dosage forms.