Development and Validation of RP-HPLC Method for Quantitative Analysis of Gabapentin in Pure and Pharmaceutical Formulations

Author(s): R. B. Desireddy, P. Jitendra Kumar, G. Naga Sowjanya, P. Prachet, Ch. Vijay Kumar, G. Suresh Kumar and K. Srinivas Rao

A validated simple, sensitive, specific and precise RP-HPLC method was developed for the determination of Gabapentin in pure and pharmaceutical formulations. Method was carried on Zodiac C18 column (250 mm × 4.6 mm × 5 µ particle size) using Methanol : Acetonitrile : Triethylamine (50 : 25 : 25) as mobile phase. Detection was carried out by U.V. at 211 nm. The proposed method obeyed linearity in the range of 10-60 µg/mL. and met all specifications as per ICH guidelines. Statistical analysis revealed that this method can be used in routine quality control studies of Gabapentin in pure and its formulations.

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