A total of 267 samples of fertile eggs containing dead-in-shell chicken embryoswere collected fromdifferent hatcheries located in Ismailia Governorate, Egypt. The percentage of positive samples was (58.8%). E.coli isolateswere isolatedwith an incidence of (10.8%), Salmonella spp. (4.4%). They were serologically identified. Pseudomonas spp. also were isolated (15.9%), Klebsiella spp. (10.8%), Proteus spp. (18.4%), Staphylococci spp.(20.3%), Streptococci spp. (12.7%) and B. anthracoid (6.3%). These isolates were biochemically identified. The penetration ability of Salmonella enterica serotype enteritidis to the intact and cracked egg shells was studied. The results revealed that it could penetrate the intact egg shells after 72 hours but it could penetrate the cracked ones after only 24 hours fromartificial contamination.