BioChemistry: An Indian Journal
ISSN (PRINT): 0974-7427

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DNA Sequencing Technologies Online Journals

DNA sequencing is any chemical, enzymatic, or technical tool used to determine the linear order of DNA nucleotide bases. Sanger sequencing in the presence of dideoxy nucleotide chain terminator monomers through replicative synthesis has now given way to 'next generation' sequencing through fast parallel read technologies. The term also refers to the entire sequence determination pipeline including post-sequencing review of the program. Sequencing DNA means the order of the four chemical building blocks that make up the DNA molecule, called "bases." Two major types of DNA sequencing are available. The older, classical method of finishing the chain is often called the Sanger method. New methods which can rapidly process a large number of DNA molecules are collectively called High-Throughput Sequencing (HTS) or Next-Generation Sequencing ( NGS) techniques. Every piece of DNA was attached to a radioactive label, and an X-ray image was taken of the gel to make the DNA band positions clear. As the DNA parts move through the gel, the sequencing machine reads and stores this information in the order of DNA bases and stores this information in its computer memory.

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Citations : 281

BioChemistry: An Indian Journal received 281 citations as per Google Scholar report

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