Abstract

The effect of cell suspension age on the result of simple spot method

Author(s): Jeanne Adiwinata Pawitan, Lia Damayanti

Aim: This study aimed to compare the quality of slides that are made from fresh suspension and those made the next day until some days afterward. Experimental: Slides were made in 5 replications directly after the cell suspension was ready, and the rest of the cell suspension was kept in the refrigerator. Further, slides were made on day 2, 3, 5 and 6 in 5 replications each. First the slides were checked for the presence of cells. The intact and damaged cells on each slide were counted, noted and tabulated. The percentage of intact cells per slide was calculated. Results: All the slides that were made directly and on day-2 contained cells, while after day-2, not all of the slides contained cells. Moreover, the slides that were made on day-6 only contained 2-4 cells per slide. The mean of the percentage of intact cells from the slides that were made directly, on day-2, -3, -5, and -6 were 65, 52, 29, 0, and 67% respectively. Conclusion: Cell suspension age has an impact on the success rate of cell containing slides and to get enough intact cells, fresh cell suspension until at last day-2 should be used.