Parası kalmadığı için otobüse binemiyordur ailesi porno izle ona daha yeni para gönderdiği için tekrar porno istemeye utanınca mecburen otostop çekmek için youporn çantasını alarak yol kenarına gelir etekli porno liseli türk kız yol kenarında dururken yanına yaklaşan porno kibar bir gencin onu gideceği yere kadar bırakmak porno izle istemesine çok mutlu olur arabaya bindiklerinde gideceği yer ile porno arabayı kullanan adamın gittiği yer arasında çok mesafe sex izle farkı olduğunu anlayan türk kız bu yaptığı porno indir iyilik karşısında arabada ona memelerini açar porno sapıklaşan adam yol kenarındaki hotelde durarak porno izle üniversiteli otostop çeken türk kızına odada sakso çektirip sikerRNase 9 Protein Inhibited Capacitation and Acrosome Reaction of Sperms| Abstract
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Abstract

RNase 9 Protein Inhibited Capacitation and Acrosome Reaction of Sperms

Author(s): Liu Jie, Yang Liping, Sun Fude, Sun chengming and Hou yuanyuan

Ribonuclease 9 is a member of ribonuclease A superfamily. It is expressed only in the epididymis. However it lacks ribonucleolytic activity and its function (s) is unknown. Immunofluorescence experiments showed that the location of RNase9 protein on sperm surfaces changed during capacitation and acrosome reaction and the protein migrated from neck to acrosome cap. CTC staining was used to assess the role of RNase 9 on capacitation and acrosome reaction. Compared with the control (PBS with 3%BSA), the spontaneous acrosome reaction induced by progesterone could be suppressed by RNase 9 protein and the AR rate is 6.5 ± 1.2%. The exposure of spermatozoa to RNase9 protein also produced a significant decrease in the intracellular cAMP levels during acrosome reaction with respect to control (111 ± 24.5 versus 187 ± 18 fmol/106 spermatozoa; P<0.05). These experiments confirmed that RNase 9 protein significantly impaired the sperm functions, including capacitation and acrosome reaction, and provide the proof for its potential in male reproductive toxicity.


Share this