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Oxidative Damage to Proteins: Spectrophotometric Method

Author(s): Kein Falasca

Protein oxidation has been the topic of a lot of research in the last ten years. Furthermore, oxygen radicals have been linked as a major contributor to protein oxidative alteration, which can lead to a fast breakdown. Carbonyl formation may be an early indication for protein oxidation among the numerous oxidative changes of amino acids in proteins. Metal catalyzed oxidation of proteins, a well-studied process, was used to describe this sort of modification. This form of protein oxidation's molecular pathways has been reviewed. In a nutshell, redox cycling cations like Fe 2+ or Cu 2+ can attach to cation binding sites on proteins and then convert side-chain amine groups on numerous amino acids to carbonyls with the help of H202 or O2.

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