Optimization of levan production from Bacillus licheniformis using response surface methodology

Author(s): Imen Dahech, Rania Bredai, Karima Srih

Optimized conditions for the levan production are necessary to increase its industrial application. This study aimed to optimize the production of levan synthesized by Bacillus licheniformis by factorial design and response surface methodology. The variables involved in this study were sucrose concentration (X1), temperature (X2), agitation (X3) and yeast extract concentration (X4). In view of the independent variables studied, the experiment showed the best results at: sucrose concentration (300 g/l), temperature 40°C, agitation 150 rpm and yeast extract concentration 3 g/l when an average production of 30.66 g/l was obtained.

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