Parası kalmadığı için otobüse binemiyordur ailesi porno izle ona daha yeni para gönderdiği için tekrar porno istemeye utanınca mecburen otostop çekmek için youporn çantasını alarak yol kenarına gelir etekli porno liseli türk kız yol kenarında dururken yanına yaklaşan porno kibar bir gencin onu gideceği yere kadar bırakmak porno izle istemesine çok mutlu olur arabaya bindiklerinde gideceği yer ile porno arabayı kullanan adamın gittiği yer arasında çok mesafe sex izle farkı olduğunu anlayan türk kız bu yaptığı porno indir iyilik karşısında arabada ona memelerini açar porno sapıklaşan adam yol kenarındaki hotelde durarak porno izle üniversiteli otostop çeken türk kızına odada sakso çektirip sikerOptimization of Carbon and Nitrogen Source in the Biotransformation Media of Baicalin by Aspergillus oryzae| Abstract
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Abstract

Optimization of Carbon and Nitrogen Source in the Biotransformation Media of Baicalin by Aspergillus oryzae

Author(s): Mei He, Wenjie Chen, Lei Tian and De-Quan Qiu

Baicalin was an important effective component in Scutellariae radix, which had extensive pharmacological activities and had been widely applied in most drugs, but it couldn’t be easily absorbed by humans. However, aglycone baicalein has better pharmacological effects and high bioavailability but with lower contents in Scutellariae radix. In this study, Aspergillus oryzae was employed as the biotransformation microorganism. And then the biotransformation of baicalin and the optimization of the carbon source and nitrogen source in the biotransformation media were conducted. Generally, the best biotransformation media of baicalin by Aspergillus oryzae was optimized as the combination of 0.0017% arginine, 0.0017% valine, 0.0017% glutamic acid, 0.0017% alanine, 0.0017% histidine, 0.0017% tyrosine and 0.01% urea, 0.4% KH2PO4, 0.04% CaCl2, and 0.04% MgSO4, pH 6, with a biotransformation efficiency of 88.36%. Compared with using corncob, bran, glucose, dissoluble starch as carbon source, it was significantly better for the biotransformation of baicalin without carbon source except the substrate baicalin. The best nitrogen source was the combination of 0.01% urea and six amino acids. Comprehensively considering biotransformation efficiency and cost, 0.1% urea was also a good inorganic nitrogen source for the biotransformation of baicalin by Aspergillus oryzae.


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