Abstract

Isolation And Characterization Of Antibacterial Peptide From Indian Cobra (Naja Naja)

Author(s): Karuna Kumar, M.K.Sachidananda, D.Channe Gowda.

Due to the development of antibiotic resistant in microorganisms, antimicrobial peptides from natural source have attracted attention in recent times. In this regard several antimicrobial peptides have been isolated from a wide range of animal source in general and snake venom in particular. Naja naja venom showed antibacterial, direct and indirect hemolytic activities. Antimicrobial peptide has been purified through gel permeation and ion exchange chromatography. The molecular mass was found to be 2491 Da by MALDI-TOF mass spectrometry and the amino acids sequence of the N-terminus was found to be DEQSTHGAYVWKL. The purified peptide showed potent antibacterial activities against gramnegative and gram-positive bacterial strains like E.coli,Pseudomonas aeruginosa, Vibrio cholarae and Staphylococcus aureus, Streptococcus faecialis, Streptococcus pnemoniae, Streptococcus pyrogenes, Bacillus subtillis respectively, comparatively most potent activity was towards gram-negative bacteria. Activity was retained at concentrations as low as 100 μg/mL. However, the peptide did not possess direct or indirect hemolytic activity.