Induction of hairy roots producing –agropine on hypocotyls explant of sunflower and tomato by Agrobacterium rhizogenes 1601 harbouring Ri- plasmid

Author(s): Raghad M.Abdullah

Transformed hairy roots were induced on Helianthus annuus and Lycopersicon esculentum by inoculation of hypocotyl explants with agropine wild type Agrobacterium rhizogenes 1601 harbouring Riplasmids. Susceptibility assessment of these plants to this strain of A. rhizogenes produced 90 and 60%transformation efficiencies respectively.. These roots were rapid negative-geotropism in their growth on agarsolidifiedMS0 mediumand having dense of root hairs. Interestingly, they spontaneously produce callus in sunflower. Paper electrophoresis of extract for both types of hairy roots demonstrated the separation of black spots of atropine.

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